Mammalian cells secrete a large number of proteins into the extracellular space, the composition and levels of which change as result of environmental factors. However, the identity of most of these secreted proteins has yet to be determined. Recent advances in proteomic technology now allow the isolation and identification of these secreted proteins. Available evidence suggests that under excitotoxic stress, neurons secrete signals that can alter the function of neurons with which they are connected via synapses or to which they are proximate. In this study we used primary neuronal cell cultures derived from the adult rat brain, and investigated the effect of chemical excitation on secreted proteins. The conditioned medium of these cultured cells was analysed using 2D-PAGE combined with MALDI-TOF mass spectrometry.
Mammalian cells secrete a large number of proteins into the extracellular space, the composition and levels of which change as result of environmental factors. However, the identity of most of these secreted proteins has yet to be determined. Recent advances in proteomic technology now allow the isolation and identification of these secreted proteins. Available evidence suggests that under excitotoxic stress, neurons secrete signals that can alter the function of neurons with which they are connected via synapses or to which they are proximate. In this study we used primary neuronal cell cultures derived from the adult rat brain, and investigated the effect of chemical excitation on secreted proteins. The conditioned medium of these cultured cells was analysed using 2D-PAGE combined with MALDI-TOF mass spectrometry.
This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters...
This volume describes prominentmethodologies developed by laboratories that have been leading the field ofquantitative proteomics by mass spectrometry. The procedures for performing theexperiments...
The aim of this book on proteomics is to provide readers an overview of the advancements in this subject. It carries descriptions of problems and conceptual challenges which are yet to be solved...
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