This volume provides acollection of protocols and approaches for the creation of novel ligand bindingproteins, compiled and described by many of today's leaders in the field ofprotein engineering. Chapters focus on modeling protein ligand bindingsites, accurate modeling of protein-ligand conformational sampling, scoring ofindividual docked solutions, structure-based design program such as ROSETTA,protein engineering, and additional methodological approaches. Examples of applicationsinclude the design of metal-binding proteins and light-induced ligand bindingproteins, the creation of binding proteins that also display catalyticactivity, and the binding of larger peptide, protein, DNA and RNAligands. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.
This volume provides acollection of protocols and approaches for the creation of novel ligand bindingproteins, compiled and described by many of today's leaders in the field ofprotein engineering. Chapters focus on modeling protein ligand bindingsites, accurate modeling of protein-ligand conformational sampling, scoring ofindividual docked solutions, structure-based design program such as ROSETTA,protein engineering, and additional methodological approaches. Examples of applicationsinclude the design of metal-binding proteins and light-induced ligand bindingproteins, the creation of binding proteins that also display catalyticactivity, and the binding of larger peptide, protein, DNA and RNAligands. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.
This volume presents a review of the latest numerical techniques used to identify ligand binding and protein complexation sites. It should be noted that there are many other theoretical studies...
A consolidated and comprehensive reference on ligand-binding assays Ligand-binding assays (LBAs) stand as the cornerstone of support for definition of the pharmaco-kinetics and toxicokinetics of...
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