This volume explores the latest advancements and techniques used by researchers to work with plant parasitic nematodes (PPNs) and to study their interactions with crop plants. The chapters in this book cover topics such as ways to obtain samples from soil and different plant tissues; rearing nematodes in greenhouse setting; methods used to axenically rear nematodes in vitro; Denaturing Gradient Gel Electrophoresis (DGGE) separation; methods for ROS detection and hydrogen peroxide quantification; methods to elicit plant resistance against nematodes; methods for the detection of Pathogenesis Related (PR) genes that are the executioners of immunity in plant-RKN interactions; and laboratory procedures that test nematodes on their isolation, count, culture, identification, pathogenicity, virulence, and environmental tolerance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plant-Nematode Interactions is a valuable tool for any researcher interested in learning more about this interesting and developing field.
This volume explores the latest advancements and techniques used by researchers to work with plant parasitic nematodes (PPNs) and to study their interactions with crop plants. The chapters in this book cover topics such as ways to obtain samples from soil and different plant tissues; rearing nematodes in greenhouse setting; methods used to axenically rear nematodes in vitro; Denaturing Gradient Gel Electrophoresis (DGGE) separation; methods for ROS detection and hydrogen peroxide quantification; methods to elicit plant resistance against nematodes; methods for the detection of Pathogenesis Related (PR) genes that are the executioners of immunity in plant-RKN interactions; and laboratory procedures that test nematodes on their isolation, count, culture, identification, pathogenicity, virulence, and environmental tolerance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plant-Nematode Interactions is a valuable tool for any researcher interested in learning more about this interesting and developing field.
This book reviews developments in the molecular biology of plant-nematode interactions that have been driven by the application of genomics tools. The book will be of interest to postgraduate...
Prologue; P.C. Sijmons. Introduction. Root Parasitic Nematodes: An Overview; U. Wyss. A: Cellular and Molecular Aspects of the Interaction. Root Anatomy and Development, The Basis for Nematode...
Nematode interactions are important biological phenomena and of great significance in agriculture. It is a fascinating subject which is multidisciplinary by nature, and concerns any scientist...
Formerly titled Plant-Parasitic Nematodes: A Pictorial Key to Genera, this volume has been the standard work on plant disease around the globe. Now in its fifth edition, it remains the fundamental...
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