1 Molecules for Gene Delivery 1-1 Leaf Huang Recent Progress in Nonviral Gene Delivery 1-2 Kenichi Yoshikawa Physical Chemistry of DNA-Carrier Complex 1-3 Kazuo Maruyama Liposome-Mediated Delivery System 1-4 Tomoko Hashimoto Polymeric Gene Carriers 1-5 Kazunori Kataoka Development of Cationic Block Copolymer-Based Gene Delivery System 1-6 Toshinori Sato Chitosan-Mediated Gene Delivery 1-7 Hidetoshi Arima Dendrimers as DNA Carriers1-8 Takuro Niidome Use of Synthetic Peptide for Non-viral Gene Delivery 1-9 Kazuo Sakurai Oligonucleotide Carrier Based on b-1,3-Glucans1-10 Mahito Nakanishi Biological and Chemical Hybrid Vectors 2 Controlled Gene Delivery 2-1 Hideyoshi Harashima Pharmacokinetics of Gene Delivery in Cells2-2 Takeshi Nagasaki Active DNA Release from Complex 2-3 Masayuki Yokoyama Controlled Release of DNA using Thermo-Responsive Polymers 2-4 Takeshi Nagasaki Active Transport of Exogenous Gene into Nucleus2-5 Masayuki Fujii Controlled Intracellular Localization of Oligonucleotides by Chemical Conjugation 2-6 Osam Mazda Functional Nucleotide Sequences Capable of Promoting Nonviral Genetic Transfer2-7 Yoshiyuki Koyama Shielding of cationic charge of the DNA complex to avoid non-specific interactions for in vivo gene delivery 2-8 Shigeru Kawakami In Vivo Gene Transfer by Ligand Modified Gene Carriers 2-9 Ernst Wagner Optimizing Polyplexes into Systemic Viruses for Tumor-Targeted Gene Therapy 2-10 Mariko Harada-Shiba Gene Transfer and Target Diseases 2-11 Hironobu Yanagie Clinical Trials Using Non-Viral Gene Delivery Systems 3 Current Protocoles of Gene Delivery 3-1 Mitsuru Hashida Evaluation of Size and Zeta-Potential of DNA/Carrier Complex 3-2 Tomoko Ito Observation of DNA / Carrier Complex under Fluorescence Microscopy 3-3 Taku Satoh Synthesis of Chemically Modified Chitosan and its Gene Transfection Efficiency 3-4 Hiroyuki Kamiya Newly Designed DNA Fragments for Gene Correcton 3-5 Tatsuya Okuda Evaluation of Gene Expression in Vivo after Intraveous and Intraportal Administration of Lipoplexes3-6 Takuro Niidome Evaluation of DNA Administrated with Gene Carrier in Organs by Southern Blot Hybridization 3-7 Fuminori Sakurai Evaluation of Immune Response after Administration of Plasmid DNA-Nonviral Vector Complex 3-8 Kunt R. Madden Gateway RNAi 4 Design of Genes Based on Current RNA Technology 4-1 Kazunari Taira Design of Intracellulary Active Ribozymes and siRNA 4-2 Takanori Yokota siRNA-based Inhibition Specific for Mutant Allele in Autosomal Dominant Diseases: Sequence-Dependent and -Independent Discriminations of Mutant and Wild-type Alleles by siRNA 4-3 Silvere Petit In Vivo RNA Interference: another tool in the box? 4-4 Hiroaki Kawasaki Suppression of Gene Expression via Chromatin Remodeling and the siRNA-Induced Silencing of Transcription 4-5 Andrew Miller Intracellular delivery of nucleic acids: differences between transfection and siFection reflect differences between DNA and RNA, and between oligodeoxynucleotides and oligonucleotides 4-6 Junichi Yano In Vivo Antitumour Activity of a New Cationic Liposome siRNA Complex 4-7 John Rossi Enhancing RNAi with Synthetic RNA Duplexes
1 Molecules for Gene Delivery 1-1 Leaf Huang Recent Progress in Nonviral Gene Delivery 1-2 Kenichi Yoshikawa Physical Chemistry of DNA-Carrier Complex 1-3 Kazuo Maruyama Liposome-Mediated Delivery System 1-4 Tomoko Hashimoto Polymeric Gene Carriers 1-5 Kazunori Kataoka Development of Cationic Block Copolymer-Based Gene Delivery System 1-6 Toshinori Sato Chitosan-Mediated Gene Delivery 1-7 Hidetoshi Arima Dendrimers as DNA Carriers1-8 Takuro Niidome Use of Synthetic Peptide for Non-viral Gene Delivery 1-9 Kazuo Sakurai Oligonucleotide Carrier Based on b-1,3-Glucans1-10 Mahito Nakanishi Biological and Chemical Hybrid Vectors 2 Controlled Gene Delivery 2-1 Hideyoshi Harashima Pharmacokinetics of Gene Delivery in Cells2-2 Takeshi Nagasaki Active DNA Release from Complex 2-3 Masayuki Yokoyama Controlled Release of DNA using Thermo-Responsive Polymers 2-4 Takeshi Nagasaki Active Transport of Exogenous Gene into Nucleus2-5 Masayuki Fujii Controlled Intracellular Localization of Oligonucleotides by Chemical Conjugation 2-6 Osam Mazda Functional Nucleotide Sequences Capable of Promoting Nonviral Genetic Transfer2-7 Yoshiyuki Koyama Shielding of cationic charge of the DNA complex to avoid non-specific interactions for in vivo gene delivery 2-8 Shigeru Kawakami In Vivo Gene Transfer by Ligand Modified Gene Carriers 2-9 Ernst Wagner Optimizing Polyplexes into Systemic Viruses for Tumor-Targeted Gene Therapy 2-10 Mariko Harada-Shiba Gene Transfer and Target Diseases 2-11 Hironobu Yanagie Clinical Trials Using Non-Viral Gene Delivery Systems 3 Current Protocoles of Gene Delivery 3-1 Mitsuru Hashida Evaluation of Size and Zeta-Potential of DNA/Carrier Complex 3-2 Tomoko Ito Observation of DNA / Carrier Complex under Fluorescence Microscopy 3-3 Taku Satoh Synthesis of Chemically Modified Chitosan and its Gene Transfection Efficiency 3-4 Hiroyuki Kamiya Newly Designed DNA Fragments for Gene Correcton 3-5 Tatsuya Okuda Evaluation of Gene Expression in Vivo after Intraveous and Intraportal Administration of Lipoplexes3-6 Takuro Niidome Evaluation of DNA Administrated with Gene Carrier in Organs by Southern Blot Hybridization 3-7 Fuminori Sakurai Evaluation of Immune Response after Administration of Plasmid DNA-Nonviral Vector Complex 3-8 Kunt R. Madden Gateway RNAi 4 Design of Genes Based on Current RNA Technology 4-1 Kazunari Taira Design of Intracellulary Active Ribozymes and siRNA 4-2 Takanori Yokota siRNA-based Inhibition Specific for Mutant Allele in Autosomal Dominant Diseases: Sequence-Dependent and -Independent Discriminations of Mutant and Wild-type Alleles by siRNA 4-3 Silvere Petit In Vivo RNA Interference: another tool in the box? 4-4 Hiroaki Kawasaki Suppression of Gene Expression via Chromatin Remodeling and the siRNA-Induced Silencing of Transcription 4-5 Andrew Miller Intracellular delivery of nucleic acids: differences between transfection and siFection reflect differences between DNA and RNA, and between oligodeoxynucleotides and oligonucleotides 4-6 Junichi Yano In Vivo Antitumour Activity of a New Cationic Liposome siRNA Complex 4-7 John Rossi Enhancing RNAi with Synthetic RNA Duplexes
The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via...
This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field ofnon-viral gene delivery vectors. The chapters in this book are...
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