This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications responsible for mitotic exit regulation; and ways to promote mitosis arrest in disease-associated conditions such as cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Mitotic Exit: Methods and Protocols is a valuable resource for all researchers interested in learning more about this important and advancing field.
This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications responsible for mitotic exit regulation; and ways to promote mitosis arrest in disease-associated conditions such as cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Mitotic Exit: Methods and Protocols is a valuable resource for all researchers interested in learning more about this important and advancing field.
This detailed book collects the main methodologies used for the analysis of the activity, localization, and regulation of the components of the Mitotic Exit Network (MEN) pathway during mitotic exit...
This volume includes a series ofprotocols focused on mitotic spindle assembly and function. The methods coveredin this book feature a broad range of techniques from basic microscopy to thestudy of...
This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work was reproduced from the original artifact, and...
Ais Endomitose wird die im natiirlichen Ablauf del' Entwicklung eines Organismus erfolgende Zweiteilung del' Chromosomen bzw. ihrer 1\qui valente im Zellkern ohne Bildung einer Spindel und ohne...
Object Lessons is a series of short, beautifully designed books about the hidden lives of ordinary things.
Exits are all around us. They are the difference between travelling and arriving, being on...
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