This volume explores the latest developments in the field and showcases the vast potential of human induced pluripotent stem cells (hiPSCs) in advancing neuroscience research. The chapters in this book are organized into three parts. Part One describes the generation, characterization, and validation of hiPSCs using different methodologies from various resources. Part Two focuses on techniques for genome editing of hiPSCs. Part Three covers various hiPSC applications in neuroscience research and strategies for analyzing hiPSC-based model systems. These applications include the generation of neural subtypes, glial cells, and skeletal muscles; assembling co-culture systems to prepare highly pure neurons at late maturation stages; measuring nuclear transport of proteins and transcripts in cultured neurons; and generating brain organoids. In the Neuromethods series style, chapters include the kind of detail and key advice from specialists needed to achieve successful results in your laboratory. Cutting-edge and comprehensive, Human Induced Pluripotent Stem Cells is a valuable resource for researchers and students in neuroscience, neurology, stem cell biology, and other related fields.
This volume explores the latest developments in the field and showcases the vast potential of human induced pluripotent stem cells (hiPSCs) in advancing neuroscience research. The chapters in this book are organized into three parts. Part One describes the generation, characterization, and validation of hiPSCs using different methodologies from various resources. Part Two focuses on techniques for genome editing of hiPSCs. Part Three covers various hiPSC applications in neuroscience research and strategies for analyzing hiPSC-based model systems. These applications include the generation of neural subtypes, glial cells, and skeletal muscles; assembling co-culture systems to prepare highly pure neurons at late maturation stages; measuring nuclear transport of proteins and transcripts in cultured neurons; and generating brain organoids. In the Neuromethods series style, chapters include the kind of detail and key advice from specialists needed to achieve successful results in your laboratory. Cutting-edge and comprehensive, Human Induced Pluripotent Stem Cells is a valuable resource for researchers and students in neuroscience, neurology, stem cell biology, and other related fields.
This detailed volume presents a series of protocols that are representative of recent developments and improvements in induced pluripotent stem cells (iPS cells) and corresponding human...
Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement,...
Induced Pluripotent Stem (iPS) cells are mature cells that have been genetically reprogrammed so that they return to their embryonic state. It is not yet known if iPS stem cells and embryonic stem...
This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the...
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