This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls. Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology.
This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls. Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology.
Furnishing the latest interdisciplinary information on the most important and frequently the only investigational system available for discovery programs that address the effects of small molecules...
High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for...
This second edition volume expands on the previous edition by exploring the latest advancements in high throughput screening (HTS) in toxicity studies by using in vitro, ex vivo, and in vivo models...
Combinatorial chemistry in conjunction with High Throughput Screening (HTS) is revolutionizing the drug discovery process. Yet, we have much to learn about the integration of these powerful...
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