I. Control of Animal Cell Growth.- Novel Specific Inhibitors for Analysis of Eukaryotic Cell Cycle Control.- II. New Cell Lines.- Transgenic Animals as a Source of Genetically-Engineered Trans-Immortalised Cell Lines.- CD8+ Suppressor T Cell Clone 13G2 Secretes a Suppressive Lymphokine, Immune Suppressive Factor-T (ISF-T).- Development, Availability and Characterization of ATCC Human and Animal Cell Lines.- III. Cell Culture System Including Serum-Free Culture.- Recent Advances in Animal Cell Biotechnology.- Critical Analysis of Process Development on in-Vitro Growth of Chick-Embryo.- Improved Method for Inoculation of a Cell Suspension into a Hollow Fiber Bioreactor.- Strategies to Increase the Efficiency of Membrane Aerated and Perfused Animal Cell Bioreactors by an Improved Medium Perfusion.- Cultivation of Hepatocytes in a New Entrapment Reactor: A Potential Bioartificial Liver.- Continuous Culture with Cell Precipitation for Recombinant Protein Production.- Elimination of Microorganisms from Cell Culture Medium Using Regenerated Cellulose Hollow Fiber (BMM).- Effects of Shear Stress on the Growth of Hybridoma Cells Cultivated in Serum-Free Medium Coupled with Ammonia Removing System.- GMP Production of Biopharmaceuticals Using High Density, Fluidized-Bed Cell Culture Technology.- Anchorage-Dependent Animal Cell Growth in Porous Microcarrier Culture.- Formation of Multicellular Aggregates of Adult Rat Hepatocytes.- Operation of an Air Lift Reactor for Production of Immunochemicals by Immobilized Hybridoma Cells.- Simulation of Growth of Hybridoma Cells Immobilized in Alginate Gel Beads Based on an Oxygen Limited Model.- High Density Suspension Culture of Insect Cells in a Stirred Bioreactor.- Media for Cultivation of Animal Cells: an Overview.- Characterization of a Human Derived Macrophage-Like Cell Line, U-M.- Mitogenic Activity from Fish Embryos and the Growth of Fish Blastocyst Cells in Culture.- Protein Factor Obtained from Rat Adipose Tissue Specifically Permits the Proliferation of 3T3-L1 and OB1771 Preadipocyte Cell Lines in a Completely Defined Serum-Free Medium.- Nutrient Optimization for the Production of Biologicals from Animal Cells Cultured at High Density.- Growth and Function of Bovine Granulosa Cells Cultured in a Serum-Free Medium.- IV. Physicochemical and Biochemical Factors for Cell Growth and Production of Biologicals.- Electrically Controlled Culture of MKN45 Cells in Serum-Free Medium.- Hybridoma Culture in the Hollow-Fiber System - the Effects of Growth Factors.- Effects of Growth Factors on Hybridoma Culture in the Perfusion System.- Studies on Physiological Aspect of In-Vitro Development of Chick-Embryo.- Pilot Scale Protein Production Using Inducible Gene Amplification.- Genetic Enhancement of Protein Productivity of Animal Cells by Oncogenes.- Immunoglobulin Production Stimulation by Various Types of Caseins.- Effect of Dilution Rate on the Metabolism and Product Formation of a Recombinant Mammalian Cell Line Growing in a Chemostat with Internal Recycle of Cells.- Immunoglobulin Production Stimulating Factors in Polysaccharides.- Production of Recombinant Protein C in a Perfusion Culture.- Optimization of Cell Culture Conditions for G-CSF (Granulocyte Colony-Stimulating Factor) Production by Genetically Engineered Namalwa KJM-1 Cells.- Variation in the Ratios and Concentrations of Nucleotide Triphosphates and UDP-Sugars during a Perfused Batch Cultivation of Hybridoma Cells.- Proteolytic Activities in Serum-Free Supernatants of Mammalian Cell Lines.- Continuous Production of Erythropoietin Using a Radial Flow Bioreactor.- V. Specific Products and Their Characterization.- Effects of Autocrine Components on Growth Inhibition of NH4+ and on Growth Kinetics of Hybridoma Cells.- Glycosylation of Erythropoietin Receptor.- Effective Purification of Monoclonal Antibodies by Fast Flow Affinity Chromatography.- VI. In Vitro Assays for Toxic, Carcinogenic, and Pharmacological Effects.- Detection of Bovi...
I. Control of Animal Cell Growth.- Novel Specific Inhibitors for Analysis of Eukaryotic Cell Cycle Control.- II. New Cell Lines.- Transgenic Animals as a Source of Genetically-Engineered Trans-Immortalised Cell Lines.- CD8+ Suppressor T Cell Clone 13G2 Secretes a Suppressive Lymphokine, Immune Suppressive Factor-T (ISF-T).- Development, Availability and Characterization of ATCC Human and Animal Cell Lines.- III. Cell Culture System Including Serum-Free Culture.- Recent Advances in Animal Cell Biotechnology.- Critical Analysis of Process Development on in-Vitro Growth of Chick-Embryo.- Improved Method for Inoculation of a Cell Suspension into a Hollow Fiber Bioreactor.- Strategies to Increase the Efficiency of Membrane Aerated and Perfused Animal Cell Bioreactors by an Improved Medium Perfusion.- Cultivation of Hepatocytes in a New Entrapment Reactor: A Potential Bioartificial Liver.- Continuous Culture with Cell Precipitation for Recombinant Protein Production.- Elimination of Microorganisms from Cell Culture Medium Using Regenerated Cellulose Hollow Fiber (BMM).- Effects of Shear Stress on the Growth of Hybridoma Cells Cultivated in Serum-Free Medium Coupled with Ammonia Removing System.- GMP Production of Biopharmaceuticals Using High Density, Fluidized-Bed Cell Culture Technology.- Anchorage-Dependent Animal Cell Growth in Porous Microcarrier Culture.- Formation of Multicellular Aggregates of Adult Rat Hepatocytes.- Operation of an Air Lift Reactor for Production of Immunochemicals by Immobilized Hybridoma Cells.- Simulation of Growth of Hybridoma Cells Immobilized in Alginate Gel Beads Based on an Oxygen Limited Model.- High Density Suspension Culture of Insect Cells in a Stirred Bioreactor.- Media for Cultivation of Animal Cells: an Overview.- Characterization of a Human Derived Macrophage-Like Cell Line, U-M.- Mitogenic Activity from Fish Embryos and the Growth of Fish Blastocyst Cells in Culture.- Protein Factor Obtained from Rat Adipose Tissue Specifically Permits the Proliferation of 3T3-L1 and OB1771 Preadipocyte Cell Lines in a Completely Defined Serum-Free Medium.- Nutrient Optimization for the Production of Biologicals from Animal Cells Cultured at High Density.- Growth and Function of Bovine Granulosa Cells Cultured in a Serum-Free Medium.- IV. Physicochemical and Biochemical Factors for Cell Growth and Production of Biologicals.- Electrically Controlled Culture of MKN45 Cells in Serum-Free Medium.- Hybridoma Culture in the Hollow-Fiber System - the Effects of Growth Factors.- Effects of Growth Factors on Hybridoma Culture in the Perfusion System.- Studies on Physiological Aspect of In-Vitro Development of Chick-Embryo.- Pilot Scale Protein Production Using Inducible Gene Amplification.- Genetic Enhancement of Protein Productivity of Animal Cells by Oncogenes.- Immunoglobulin Production Stimulation by Various Types of Caseins.- Effect of Dilution Rate on the Metabolism and Product Formation of a Recombinant Mammalian Cell Line Growing in a Chemostat with Internal Recycle of Cells.- Immunoglobulin Production Stimulating Factors in Polysaccharides.- Production of Recombinant Protein C in a Perfusion Culture.- Optimization of Cell Culture Conditions for G-CSF (Granulocyte Colony-Stimulating Factor) Production by Genetically Engineered Namalwa KJM-1 Cells.- Variation in the Ratios and Concentrations of Nucleotide Triphosphates and UDP-Sugars during a Perfused Batch Cultivation of Hybridoma Cells.- Proteolytic Activities in Serum-Free Supernatants of Mammalian Cell Lines.- Continuous Production of Erythropoietin Using a Radial Flow Bioreactor.- V. Specific Products and Their Characterization.- Effects of Autocrine Components on Growth Inhibition of NH4+ and on Growth Kinetics of Hybridoma Cells.- Glycosylation of Erythropoietin Receptor.- Effective Purification of Monoclonal Antibodies by Fast Flow Affinity Chromatography.- VI. In Vitro Assays for Toxic, Carcinogenic, and Pharmacological Effects.- Detection of Bovi...
This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then...
Over the past ten years several sophisticated in vitro test systems based on epithelial cell cultures have been introduced in the field of drug delivery. These models have been found to be very...
Commercial development of cultured-derived food ingredients has attracted interna tional interest. As consumers have become more health conscious in recent years, the de mand for natural food...
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