Poultry farming is the most dynamic & 2nd major industry in Pakistan. It has challenges of outbreaks of viral diseases resulting in high mortality, morbidity and huge economic loss in Pakistan. Conventional diagnostic tests are expensive, laborious & require technical expertise. Hence, a highly sensitive & specific dot-ELISA kit is developed, in two steps; first whole antigen is prepared from AIV, NDV and IBV and is applied onto nitrocellulose membrane; second rabbit anti-chicken antibodies is raised, purified and conjugated with enzyme HRP. Dot ELISA results are much comparative with ELISA & have advantages of high specificity, sensitivity & facile. This kit has the advantage of loading multiple antigens from local isolates & can be performed even at poultry forms; with no technical expertise or advance laboratory facilities for interpretations. Even the staff can test their bird's immune response after vaccination or test for the presence of infection. It can be prepared & used on large scale; this combine approach of several antigens on single strip play a vital role in the diagnosis of poultry diseases or determining immune status and help in controlling diseases in Pakistan.
Poultry farming is the most dynamic & 2nd major industry in Pakistan. It has challenges of outbreaks of viral diseases resulting in high mortality, morbidity and huge economic loss in Pakistan. Conventional diagnostic tests are expensive, laborious & require technical expertise. Hence, a highly sensitive & specific dot-ELISA kit is developed, in two steps; first whole antigen is prepared from AIV, NDV and IBV and is applied onto nitrocellulose membrane; second rabbit anti-chicken antibodies is raised, purified and conjugated with enzyme HRP. Dot ELISA results are much comparative with ELISA & have advantages of high specificity, sensitivity & facile. This kit has the advantage of loading multiple antigens from local isolates & can be performed even at poultry forms; with no technical expertise or advance laboratory facilities for interpretations. Even the staff can test their bird's immune response after vaccination or test for the presence of infection. It can be prepared & used on large scale; this combine approach of several antigens on single strip play a vital role in the diagnosis of poultry diseases or determining immune status and help in controlling diseases in Pakistan.
Questo studio ha incluso cinque antigeni, ciascuno esaminato con tre immunoglobuline (IgG totali, IgG1 e IgG4) utilizzando il metodo ELISA a titolazione a scacchiera per determinare la concentrazione...
Este estudo incluiu cinco antigénios, cada um examinado com três imunoglobulinas (IgG total, IgG1 e IgG4) utilizando o ELISA de titulação em tabuleiro de xadrez para determinar a concentração ideal...
This volume provides an understanding of how an immunoassay works, detailing the strengths, weaknesses, pitfalls. Chapters guide readers on how and when to appropriately utilize this powerful...
What could be more fun than a tea party?"A polka dot tea party of course," says Jackie Rabbit.This easy-to-read book is about friendship and discovery. It contains colorful illustrations...
This book contains a year's worth of writing. Themes I touch upon include, but are not limited to: society, autobiographical, deafness and language, beauty and love, and a bunch of nonsense.
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